报告题目：Microfluidics in cell culture and gene therapy
Ciprian Iliescu博士，罗马尼亚人，现任新加坡国立大学“BIGHEART”生物研究中心研究员。于1999年在罗马尼亚布加勒斯特理工大学获博士学位，2001年到2003年在新加坡南洋理工大学做博士后。之后在新加坡生物工程和纳米技术研究所（IBN）工作十多年里，主要从事基于MEMS技术的organs on a chip, electrical impedance spectroscopy, microfluidics, isolation of the circulating tumor cells, transdermal drug delivery, DNA compaction for gene therapy等交叉学科研究。Ciprian Iliescu博士迄今为止已在著名期刊发表学术论文约158篇，H因子33。
Microfluidics in cell culture and gene therapy
Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, National University of Singapore (Singapore)
Two topics of microfluidics will be covered: organ on a chip, and DNA compaction for gene therapy applications.
The present work underlines the main achievements of Dr. Iliescu in the field: a 3D culture model named constrain spheroids (CS) that can operate in both static or perfusion culture. In this model the cells aggregate in spheroids which are stabilized in a sandwich configuration between surface modified glass slide and a microfabricated ultra-thin Parylene C membrane maximizing mass transfer, and overcome problems with uneven cell count and spheroids size.
Dr. Iliescu will then illustrate how microfluidic hydrodynamic flow focusing enables an accurate control of self-assembling nanoparticles with emphasis on DNA compaction. Two approaches will be illustrated. The first method is based on rapid change of solvent quality and the second one relies on the controlled diffusive mixing of surfactant and DNA solutions through a water stream of tunable width. These microfluidic methods can be successfully used for gene therapy applications as well as for the synthesis of tailored soft nanoparticles.